Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems
ثبت نشده
چکیده
In this study we compare the efficiency of different blotting techniques, including semi-dry, tank transfer, and fast-blotting systems on proteins across a wide range of molecular weights with particular emphasis on large proteins (≥ 200 kD). We also conducted experiments transferring high molecular weight proteins that are difficult to transfect using conventional methods. The results demonstrate that the transfer efficiency of the Trans Blot Turbo transfer system meets or exceeds that of other comparable techniques.
منابع مشابه
Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems
In this study we compare the efficiency of different blotting techniques, including semi-dry, tank transfer, and fast blotting systems on proteins across a wide range of molecular weights with particular emphasis on large proteins (≥200 kD). We also conducted experiments transferring high molecular weight proteins that are difficult to transfect using conventional methods. The results demonstra...
متن کاملPmn-19: A Comparison of Neonatal Outcomes following Transfer of Frozen and Fresh Embryo
Background: Currently, frozen embryo transfers contribute an increasing rate of births from assisted reproductive technologies such as in vitro fertilization (IVF). However, very few studies evaluated the neonatal outcomes from frozen embryo transfer. This study aimed to investigate and compare the neonatal outcomes from frozen embryo transfer versus fresh embryo transfers. Materials and Method...
متن کاملModeling of Reversible Chain Transfer Catalyzed Polymerization by Moment Equations Method
A moment equations method was performed to study the Reversible chain Transfer Catalyzed Polymerization (RTCP) of styrene in 80°C. To do this, a kinetic scheme containing conventional free radical polymerization reactions and equilibrium reactions of RTCP was assumed. After obtaining mass balance equations, three moment equations were defined for free and dormant radicals and dead chains. M...
متن کاملSynthesis and identification of polystyrene via conventional and controlled radical polymerization methods: Effect of temperature, initiator and transfer agent on molecular weight and reaction rate
Polystyrene (PSt) has been known as one of the important polymers with a wide range of applications. Ability to synthesize PSt with different but predictable molecular weights for various applications is very important in the laboratories and industries. In this study, using various simple and inexpensive techniques with only free radical mechanism, it was tried to synthesize PSt with different...
متن کاملAnalytical determination of the chemical exchange saturation transfer (CEST) contrast in molecular magnetic resonance imaging
Magnetic resonance based on molecular imaging allows tracing contrast agents thereby facilitating early diagnosis of diseases in a non-invasive fashion that enhances the soft tissue with high spatial resolution. Recently, the exchange of protons between the contrast agent and water, known as the chemical exchange saturation transfer (CEST) effect, has been measured by applying a suitable pulse ...
متن کامل